食品残渣扩繁降解菌对园林废弃物堆肥碳转化的影响

张陆; 王宏戈; 王惟帅; 王选; 李静; 李琳; 郭伟婷; 刘双; 王红; 陆云丽; 马林

doi:10.12357/cjea.20220744

食品残渣扩繁降解菌对园林废弃物堆肥碳转化的影响

doi: 10.12357/cjea.20220744

张陆^{1, 2,},
王宏戈^{1, 2},
王惟帅¹,
王选^{1, 3},
李静⁴,
李琳⁵,
郭伟婷⁶,
刘双⁷,
王红⁷,
陆云丽⁸,
马林^{1, 2, ,}

1.
中国科学院遗传与发育生物学研究所农业资源研究中心/河北省土壤生态学重点实验室/中国科学院农业水资源重点实验室　石家庄　050022
2.
中国科学院大学　北京　100049
3.
中国科学院雄安创新研究院　雄安　071700
4.
石家庄市动物园　石家庄　050299
5.
石家庄市植物园　石家庄　050200
6.
石家庄市畜牧技术推广站　石家庄　050030
7.
河北省畜牧总站　石家庄　050030
8.
河北绿色永续环保科技有限公司　石家庄　050061

基金项目: 河北省重点研发计划项目(20373806D)、中国科学院科技服务网络计划项目(KFJ-STS-QYZD-160)、中国科学院青年创新促进会(2021095)、河北省现代农业产业技术体系奶牛产业创新团队项目(HBCT2018120206)和河北省现代农业产业技术体系蛋肉鸡产业创新团队项目(HBCT2018150209)资助

详细信息

作者简介:
张陆, 主要从事农业生态学研究。E-mail: 164192510@qq.com

通讯作者:
马林, 主要从事农业生态学和养分管理研究。E-mail: malin1979@sjziam.ac.cn

中图分类号: X712
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- 文章访问数: 123
- HTML全文浏览量: 78
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- 被引次数: 0
出版历程
- 收稿日期: 2022-09-28
- 录用日期: 2022-11-03
- 网络出版日期: 2022-11-25
- 刊出日期: 2023-05-10

Accelerating carbon conversion in garden waste composting with food waste-expanding microbial inoculants

ZHANG Lu^{1, 2
,},
WANG Hongge^{1, 2},
WANG Weishuai¹,
WANG Xuan^{1, 3},
LI Jing⁴,
LI Lin⁵,
GUO Weiting⁶,
LIU Shuang⁷,
WANG Hong⁷,
LU Yunli⁸,
MA Lin^{1, 2
, ,}

1.
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences / Hebei Key Laboratory of Soil Ecology / Key Laboratory of Agricultural Water Resources, Chinese Academy of Sciences, Shijiazhuang 050022, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China
3.
Xiong’an Innovation Research Institute, Chinese Academy of Sciences, Xiong’an 071700, China
4.
Shijiazhuang Zoo, Shijiazhuang 050299, China
5.
Shijiazhuang Botanical Garden, Shijiazhuang 050200, China
6.
Shijiazhuang Animal Husbandry Technology Extension Station, Shijiazhuang 050030, China
7.
Hebei Provincial Animal Husbandry Station, Shijiazhuang 050030, China
8.
Hebei Green Sustainable Environmental Protection Technology Co., Ltd., Shijiazhuang 050061, China

Funds: This study was supported by the Key Research and Development Program of Hebei Province (20373806D), the Science and Technology Service Network Program of Chinese Academy of Sciences (KFJ-STS-QYZD-160), the Youth Innovation Promotion Association of Chinese Academy of Sciences (2021095), Hebei Province Modern Agricultural Industrial Technology System Dairy Cow Industry Innovation Team Project (HBCT2018120206), Hebei Province Modern Agricultural Industrial Technology System Egg Broiler Industry Innovation Team Project (HBCT2018150209).

More Information

Corresponding author: E-mail: malin1979@sjziam.ac.cn

摘要

摘要: 高木质纤维素含量制约了园林废弃物的堆肥化应用, 添加外源菌剂是加快木质纤维素降解的有效手段。为降低菌剂生产成本并提高接种效率, 本研究利用食品残渣(苹果渣、豆渣)代替常规碳氮源(葡萄糖、蛋白胨)进行木质纤维素降解菌的扩繁, 扩繁产品活菌数高达3.7×10¹⁰ cfu∙mL⁻¹, 相较工业培养基增加46.2%。进一步探讨了不同降解菌接种量(0、2%、4%、8%, 干重计)对园林废弃物堆肥过程碳素转化的影响。结果表明, 接种处理显著增加了木质纤维素的降解(P<0.05), 2%、4%和8%接种处理(2%IM、4%IM、8%IM)的总木质纤维素降解率较CK分别提高6.3%、9.2%和23.0%; 其中8%IM处理加速了碳素的完全矿化, 导致腐殖质前体物(多酚、还原糖)被完全降解生成CO₂, 抑制了腐殖化的进行; 而4%IM处理在加快木质纤维素降解的同时促进了腐殖酸(HA)的合成, 其最终HA含量达91.3 g∙kg⁻¹, 较CK、2%IM和8%IM处理分别提高24.9%、10.7%和35.8%。因此, 以食品残渣为培养基质可完全实现木质纤维素降解菌的生长扩繁, 同时, 4%接种量更有利于园林废弃物堆肥腐殖化的进行和碳素的保存, 本研究为多源废弃物高效协同处理提供了理论依据。
- 堆肥 /
- 园林废弃物 /
- 碳素转化 /
- 食品残渣 /
- 木质纤维素降解 /
- 腐殖化
Abstract: The expansion of urbanization has resulted in the generation of a large amount of garden waste (40 million tons per year in China), while traditional treatment methods (incineration and landfill) tend to cause serious environmental pollution and waste of resources. Composting is an effective way to realize resource utilization of garden waste. However, the high lignocellulose content of garden waste limits its resource utilization. Accelerating the degradation of lignocellulose in the composting process is of great significance for achieving effective resource utilization of garden waste. Inoculation with exogenous microorganisms is considered an environmentally friendly and cost-effective method to accelerate lignocellulose degradation, which would further reduce the cost of inoculum production and improve inoculation efficiency. In this study, food residues (apple pomace and bean dregs) were used instead of conventional carbon and nitrogen sources (glucose and peptone) to propagate lignocellulose-degrading fungi. The number of viable fungi in the multiplication product reached 3.7×10¹⁰ cfu∙mL⁻¹, which increased by 46.2% compared with the traditional industrial medium. The effects of different inoculum amounts (0, 2%, 4%, and 8%, dry weight) on carbon conversion during garden waste composting were also discussed. The inoculation treatments significantly increased lignocellulose degradation (P<0.05), according to the results. The total lignocellulose degradation rates of the 2%, 4%, and 8% inoculation treatments (2%IM, 4%IM, and 8%IM) increased by 6.3%, 9.2%, and 23.0%, respectively, compared with CK. Dynamic changes in humus precursors (reducing sugars and polyphenols) and humus components were further analyzed. The 8%IM treatment accelerated the complete mineralization of carbon, resulting in the complete degradation of the humus precursors (polyphenols and reducing sugars) into CO₂, which inhibited humification. Compared with CK, 2%IM, and 4%IM, the cumulative CO₂ emissions of 8%IM increased by 21.9%, 22.3%, and 26.0%, respectively. The 4%IM treatment accelerated lignocellulose degradation while promoting the synthesis of humic acid (HA). The final HA content reached 91.3 g∙kg⁻¹, which was 24.9%, 10.7%, and 35.8% higher than that of CK, 2%IM, and 8%IM treatments, respectively. These results indicate that appropriate inoculation is beneficial to the directional transformation of lignocellulose to humic acid, whereas excessive inoculation would lead to an excessive loss of organic matter due to the high metabolic activity of microorganisms; and the degradation efficiency of lignocellulose is lower when inoculated with a small amount, which was further confirmed by the partial least squares path analysis model in this study. The conversion of lignocellulose to dissolved organic carbon increased with increasing inoculation amount (correlation coefficients of CK, 2%IM, 4%IM, and 8%IM were 0.59, 0.70, 0.75, and 0.85, respectively), while the correlation coefficient of 4%IM from DOC to HA was −0.85, which was higher than 2%IM (−0.76) and 8%IM (−0.34). Therefore, the growth and propagation of lignocellulose-degrading fungi can be completely realized by using food residues as a culture substrate. A 4% inoculation amount was more conducive to the humification of garden waste compost and the preservation of carbon. This study provides a reference for the garden waste composting inoculation process and a theoretical basis for multi-source waste-efficient collaborative treatment.
- Composting /
- Garden waste /
- Carbon transformation /
- Food waste /
- Lignocellulose degradation /
- Humification

HTML全文

图 1 接种量对堆肥过程木质纤维素降解的影响

CK: 对照; 2%IM: 添加2%菌剂; 4%IM: 添加4%菌剂; 8%IM: 添加8%菌剂; 不同小写字母表示不同处理间存在显著性差异(P<0.05)。CK: control; 2%IM: adding 2% microbial inoculum; 4%IM: adding 4% microbial inoculum; 8%IM: adding 8% microbial inoculum. Different lowercase letters indicate significant differences among different treatments (P<0.05)

Figure 1. Effects of inoculum size on lignocellulose degradation during composting

下载: 全尺寸图片幻灯片

图 2 接种量对堆肥过程水溶性碳组分的影响

CK: 对照; 2%IM: 添加2%菌剂; 4%IM: 添加4%菌剂; 8%IM: 添加8%菌剂。CK: control; 2%IM: adding 2% microbial inoculum; 4%IM: adding 4% microbial inoculum; 8%IM: adding 8% microbial inoculum.

Figure 2. Effects of inoculum size on water-soluble carbon components during composting

下载: 全尺寸图片幻灯片

图 3 接种量对堆肥过程中碳素矿化的影响

CK: 对照; 2%IM: 添加2%菌剂; 4%IM: 添加4%菌剂; 8%IM: 添加8%菌剂。CK: control; 2%IM: adding 2% microbial inoculum; 4%IM: adding 4% microbial inoculum; 8%IM: adding 8% microbial inoculum.

Figure 3. Effect of inoculum size on carbon mineralization during composting

下载: 全尺寸图片幻灯片

图 4 接种量对堆肥过程中腐殖化的影响

CK: 对照; 2%IM: 添加2%菌剂; 4%IM: 添加4%菌剂; 8%IM: 添加8%菌剂。CK: control; 2%IM: adding 2% microbial inoculum; 4%IM: adding 4% microbial inoculum; 8%IM: adding 8% microbial inoculum.

Figure 4. Effect of inoculum size on humification during composting

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图 5 接种量对堆肥碳素转化途径的影响

DOC: 可溶性有机碳; Lce: 木质纤维素; HA: 腐殖酸。CK: 对照; 2%IM: 添加2%菌剂; 4%IM: 添加4%菌剂; 8%IM: 添加8%菌剂。DOC: dissolved organic carbon; Lce: lignocellulose; HA: humic acid. CK: control; 2%IM: adding 2% microbial inoculum; 4%IM: adding 4% microbial inoculum; 8%IM: adding 8% microbial inoculum.

Figure 5. Effects of inoculum size on carbon conversion pathway during composting

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表 1 试验材料的理化性状

Table 1. Physical and chemical properties of the experimental materials

试验材料 Material	含水率 Moisture content (%)	有机质^a Organic matter (%)	总氮^a Total nitrogen (%)	碳氮比^a C/N	pH	电导率 Electrical conductivity (mS·cm⁻¹)	总糖^a Total sugar content (%)
园林废弃物 Garden waste	11.00±0.34	84.74±0.56	1.49±0.00	34.14±0.20	7.32±0.02	1.55±0.17	—
餐厨垃圾 Food waste	78.55±2.23	91.74±0.11	2.89±0.02	14.61±0.32	4.58±0.07	3.30±0.20	—
苹果渣 Apple pomace	82.81±0.58	98.04±0.14	0.81±0.02	69.87±1.82	5.67±0.09	—	20.75±0.58
豆渣 Bean dregs	79.95±4.14	95.73±0.07	2.64±0.01	21.02±0.09	7.07±0.04	—	37.29±1.29
“a”: 基于物料干重; “—”: 表示未检测。“a”: based on dry weight of material; “—”: not measured.

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表 2 食品残渣(苹果渣、豆渣)代替常规碳、氮源(培养基 Ⅱ)对培养72 h后菌液活菌数与酶活性的影响

Table 2. Effect of food residues (apple pomace, soybean dregs) instead of conventional carbon and nitrogen sources (Medium Ⅱ) on the viable count and enzymatic activity of the broth after 72 h incubation

处理 Treatment	活菌数 Viable count (×0¹⁰ cfu∙mL⁻¹)	木聚糖酶 Xylanase (U∙mL⁻¹)	纤维素酶 Cellulase (U∙mL⁻¹)	漆酶 Laccase (U∙mL⁻¹)	锰过氧化物酶 Manganese peroxidase (U∙mL⁻¹)	木质素过氧化物酶 Lignin peroxidase (U∙mL⁻¹)
培养基Ⅰ Medium Ⅰ	2.53±0.41a	337.40±108.81a	73.82±9.55a	3.71±0.69a	11.26±3.97a	2.98±1.67a
培养基Ⅱ Medium Ⅱ	3.70±0.37b	597.40±171.21b	99.47±5.99b	4.91±2.22a	22.38±2.46b	12.39±1.67b
不同小写字母表示不同处理间存在显著性差异(P<0.05)。Different lowercase letters indicate significant differences between two treatments (P<0.05).

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