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万年县古稻原产区细菌多样性分析及功能预测

刘亚军 汪成钵 章涛 叶翠 储小东 廖文成 李荣富 吴永明

刘亚军, 汪成钵, 章涛, 叶翠, 储小东, 廖文成, 李荣富, 吴永明. 万年县古稻原产区细菌多样性分析及功能预测[J]. 中国生态农业学报 (中英文), 2023, 31(0): 1−11 doi: 10.12357/cjea.20230448
引用本文: 刘亚军, 汪成钵, 章涛, 叶翠, 储小东, 廖文成, 李荣富, 吴永明. 万年县古稻原产区细菌多样性分析及功能预测[J]. 中国生态农业学报 (中英文), 2023, 31(0): 1−11 doi: 10.12357/cjea.20230448
LIU Y J, WANG C B, ZHANG T, YE C, CHU X D, LIAO W C, LI R F, WU Y M. Bacterial diversity exploring and functional prediction in ancient rice original-producing regions of Wannian County, China[J]. Chinese Journal of Eco-Agriculture, 2023, 31(0): 1−11 doi: 10.12357/cjea.20230448
Citation: LIU Y J, WANG C B, ZHANG T, YE C, CHU X D, LIAO W C, LI R F, WU Y M. Bacterial diversity exploring and functional prediction in ancient rice original-producing regions of Wannian County, China[J]. Chinese Journal of Eco-Agriculture, 2023, 31(0): 1−11 doi: 10.12357/cjea.20230448

万年县古稻原产区细菌多样性分析及功能预测

doi: 10.12357/cjea.20230448
基金项目: 江西省科学院省级科研项目经费包干制试点示范项目(2023YSBG22012, 2021YSBG22030)、江西省科学院引进博士项目(2023YYB01)、江西省地质调查勘查院科研资金项目(赣地调字[2023]11号)资助
详细信息
    通讯作者:

    吴永明,主要从事流域生态环境研究。E-mail: 7086006@qq.com

  • 中图分类号: X172

Bacterial diversity exploring and functional prediction in ancient rice original-producing regions of Wannian County, China

Funds: This study was supported by Jiangxi Academy of Sciences Provincial Science and Technology Plan Project Contract System Pilot Demonstration Project (2023YSBG22011, 2021YSBG22030), Jiangxi Academy of Sciences PhD Introduction Project (2023-YYB-01) and Jiangxi Geological Survey and Exploration Institute Research Fund Project ([2023]11).
More Information
  • 摘要: 为探讨我国古稻原产区独特的土壤微生态环境, 本研究以江西省万年县古稻原产区及临近区域稻田为研究对象, 采用16S rDNA高通量测序技术和FAPROTAX功能预测分析了细菌群落结构及功能, 并探究影响细菌群落结构与功能特性的关键土壤理化因子。结果显示, 古稻原产区土壤除了包含更高含量的有效氮和Cu, 其他理化因子与临近区域并未表现出明显的差异。古稻原产区的细菌群落特征与其他区域的差异性主要体现在种群组成上, 而不是细菌丰度(基于16S rDNA 荧光定量PCR技术)和Alpha多样性。与临近区域相比, 古稻原产区土壤中包含更高相对丰度的厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidota), 酸杆菌门(Acidobacteriota)和硝化螺旋菌门(Nitrospirota)则相反。此外, 古稻原产区细菌类群表现出更强的碳代谢(包括甲醇氧化、发酵、纤维素分解和碳氢化合物降解等)能力和更弱的氮(包括硝化作用和N2O反硝化等)、硫代谢潜力。进一步的分析发现, 细菌的群落和功能潜力特征除了受土壤营养元素的影响, 还受pH和多种重金属元素(如Cd、Cu、Hg、Ni)的影响。本研究解析了古稻原产区微生态环境的特异性, 明确了其稻田土壤拥有更高的碳周转和氮储能力, 为未来推广优质水稻种植和改善农田生态系统提供了理论参考。
  • 图  1  研究区域及样点分布图

    Figure  1.  Locations of study area and sampling sites

    图  2  不同区域细菌群落的基于OTU水平的主坐标分析(a)、基于Adonis的组间差异分析(b)和基于Bray-curtis距离的物种组内差异分析(c)

    ZTN: 珠田北; ZTS: 珠田南; SFE: 上坊东; SFW: 上坊西; PME: 裴梅东; PMW: 裴梅西。ZTN: north of Zhutian Township; ZTS: south of Zhutian Township; SFE: east of Shangfang Township; SFW: west of Shangfang Township; PME: west of Peimei Township; PMW: east of Peimei Township.

    Figure  2.  (a) ordination of soil bacterial communities via principal coordinate analysis (PCoA) of bacterial communities at the OTU level; (b) analysis of differences between groups based on Adonis; (c) analysis of differences within species groups based on Bray-curtis distances.

    图  3  不同区域水稻土壤优势(>1%)细菌门(a)和属(b)水平的相对丰度

    ZTN: 珠田北; ZTS: 珠田南; SFE: 上坊东; SFW: 上坊西; PME: 裴梅东; PMW: 裴梅西。ZTN: north of Zhutian Township; ZTS: south of Zhutian Township; SFE: east of Shangfang Township; SFW: west of Shangfang Township; PME: west of Peimei Township; PMW: east of Peimei Township.

    Figure  3.  Relative abundances of the soil bacterial communities of paddy in different areas at the dominant (>1%) phylum (a) and genus (b)

    图  4  不同研究区土壤微生物菌群预测功能分组

    ZTN: 珠田北; ZTS: 珠田南; SFE: 上坊东; SFW: 上坊西; PME: 裴梅东; PMW: 裴梅西。ZTN: north of Zhutian Township; ZTS: south of Zhutian Township; SFE: east of Shangfang Township; SFW: west of Shangfang Township; PME: west of Peimei Township; PMW: east of Peimei Township.

    Figure  4.  Functional prediction of the microbial flora in different study areas.

    图  5  细菌群落结构、功能与土壤性质的关系

    Clay: 黏粒; Silt: 粉粒; Sand: 砂粒; CEC: 阳离子交换量; OM: 有机质; TN: 总氮; TP: 总磷; AN: 有效氮; AK: 有效钾; AP: 有效磷。CEC: cation exchange capacity; OM: organic matter; TN: total nitrogen; TP: total phosphorus; AN: available nitrogen; AK: available potassium; AP: available phosphorus.

    Figure  5.  Relationship between bacterial communities, functional and soil properties

    表  1  不同研究区域土壤理化指标

    Table  1.   Physicochemical parameters of soil samples from different areas

    指标
    Index
    附近普通稻产区
    Normal rice growing regions nearby
    古稻原产区
     Ancient rice original
    producing region
    ZTN (珠田北)ZTS (珠田南)SFE (上坊东)SFW (上坊西)PME (裴梅东)PMW (裴梅西)
    黏粒 Clay (%)5.45±2.93b2.75±1.75b4.57±1.79b0.69±0.62b31.36±15.55a2.1±1.39b
    粉粒 Silt (%)59.52±8.10a63.02±10.02a62.02±8.44a61.62±10.66a50.85±11.32a69.3±4.6a
    砂粒 Sand (%)35.03±9.57a34.23±9.66a33.41±7.64a37.69±11.12a17.79±5.32a28.6±5.08a
    pH5.45±0.29a5.4±0.14a5.14±0.07a5.22±0.08a5.24±0.07a5.13±0.07a
    阳离子交换量
    Cation exchange capacity (cmol∙kg−1)
    8.21±0.36a9.65±1.33a7.34±0.39a7.49±0.30a8.06±0.63a9.40±0.35a
    有机质 Organic matter (g∙kg−1)24.79±6.32a37.74±5.35a28.67±4.44a34.58±3.36a32.05±8.21a31.10±3.85a
    总氮 Total nitrogen (g∙kg−1)1.19±0.18a1.99±0.35a1.67±0.28a2.17±0.13a1.95±0.53a2.41±0.20a
    总磷 Total phosphorus (g∙kg−1)0.56±0.07a0.58±0.06a0.54±0.04a0.46±0.06a0.44±0.07a0.51±0.05a
    有效氮 Available nitrogen (mg∙kg−1)106.93±16.53b160.85±23.00b137.87±22.56b177.82±7.00b154.19±37.94b289.57±41.24a
    有效钾 Available potassium (mg∙kg−1)114.70±27.33a93.38±27.86a105.92±19.84a112.58±19.74a113.45±12.21a109.79±16.04a
    有效磷 Available phosphorus (mg∙kg−1)21.77±2.46a16.78±2.96a13.59±1.10a18.41±3.98a16.15±2.70a15.69±1.19a
    镉 Cd (mg∙kg−1)0.27±0.02a0.17±0.01b0.15±0.01ab0.27±0.05a0.17±0.02ab0.16±0.01ab
    铬 Cr (mg∙kg−1)44.4±5.66a43.2±5.43a45.6±4.18a42.0±1.26a58.6±3.17a44.2±3.01a
    铅 Pb (mg∙kg−1)27.82±3.54a30.34±5.71a33.00±5.32a32.40±2.96a32.00±4.53a32.40±3.64a
    锌 Zn (mg∙kg−1)80.2±9.3ab97.6±5.31ab83.6±6.72ab103.2±11.00a80.6±10.64ab64.6±3.89b
    铜 Cu (mg∙kg−1)26.0±5.19b34.8±5.81b40.8±5.03ab45.0± 4.44ab41.8±5.95ab57.6±5.37a
    汞 Hg (mg∙kg−1)0.14±0.04a0.07±0.01a0.08±0.01a0.08±0.01a0.08±0.01a0.08±0.01a
    镍 Ni (mg∙kg−1)30.8±6.19a41.4±3.19a36.8±2.82a45.4±1.44a31.8±1.85a32.4±3.31a
    砷 As (mg∙kg−1)2.79±0.74a2.43±0.81a2.36±1.07a1.72±0.26a1.78±0.28a1.86±0.3a
      数据为平均值±标准误, 同行不同字母表示差异显著(P<0.05)。Values are mean±SD. Different lowercase letters in each row are significantly different (P<0.05).
    下载: 导出CSV

    表  2  不同研究区域细菌群落丰度及多样性

    Table  2.   Bacterial community abundance and diversity of soil samples from different areas

    指标
    Index
    附近普通稻产区
    Normal rice growing regions nearby
    古稻原产区
     Ancient rice original
    producing region
    ZTN (珠田北)ZTS (珠田南)SFE (上坊东)SFW (上坊西)PME (裴梅东)PMW (裴梅西)
    细菌丰度
    Bacterial abundance (1010 copies∙g−1)
    1.91±0.36a3.22±0.56a2.22±0.46a2.62±0.48a1.84±0.53a2.51±0.19a
    物种丰富度指数
    ACE index
    5042.44±759.8a5268.53±374.29a6168.65±266.56a6256.8±126.56a5529.5±140.48a6055.51±182.27a
    物种丰富度指数
    Chao1 index
    4995.17±739.35a5238.03±380.39a6048.62±278.81a6147.41±117.5a5486.65±128.56a5938.04±193.99a
    香农-威纳指数
    Shannon index
    6.91±0.27a7.12±0.06a7.34±0.09a7.27±0.04a7.24±0.04a7.28±0.05a
    辛普森指数
    Simpson index
    0.0041±0.0013a0.0023±0.0001a0.002±0.0003a0.0022±0.0002a0.002±0.0001a0.0024±0.0001a
    谱系多样性指数
    PD_whole_tree index
    190.17±24.76a205.71±10.91a230.53±7.39a233.82±4.48a215.89±4.44a233.64±5.76a
      数据为平均值±标准误, 同行不同小写字母表示差异显著(P<0.05)。Values are mean±SD. Different lowercase letters in each row are significantly different (P<0.05).
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-08-16
  • 录用日期:  2023-10-23
  • 修回日期:  2023-10-23
  • 网络出版日期:  2023-11-01

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