杨小环, 杨婧怡, 王子然, 王成龙, 宋亚萍, 马金虎. 六价铬对红芸豆种子萌发和幼苗生长的毒害作用及外源NO的缓解效应[J]. 中国生态农业学报 (中英文), 2024, 32(0): 1−11. DOI: 10.12357/cjea.20240007
引用本文: 杨小环, 杨婧怡, 王子然, 王成龙, 宋亚萍, 马金虎. 六价铬对红芸豆种子萌发和幼苗生长的毒害作用及外源NO的缓解效应[J]. 中国生态农业学报 (中英文), 2024, 32(0): 1−11. DOI: 10.12357/cjea.20240007
YANG X H, YANG J Y, WANG Z R, WANG C L, SONG Y P, MA J H. Toxic effect of hexavalent chromium on seed germination and seedling growth of red kidney bean and the alleviation effect of exogenous NO[J]. Chinese Journal of Eco-Agriculture, 2024, 32(0): 1−11. DOI: 10.12357/cjea.20240007
Citation: YANG X H, YANG J Y, WANG Z R, WANG C L, SONG Y P, MA J H. Toxic effect of hexavalent chromium on seed germination and seedling growth of red kidney bean and the alleviation effect of exogenous NO[J]. Chinese Journal of Eco-Agriculture, 2024, 32(0): 1−11. DOI: 10.12357/cjea.20240007

六价铬对红芸豆种子萌发和幼苗生长的毒害作用及外源NO的缓解效应

Toxic effect of hexavalent chromium on seed germination and seedling growth of red kidney bean and the alleviation effect of exogenous NO

  • 摘要: 为了研究铬(Cr)对红芸豆种子萌发和早期幼苗生长的胁迫作用及硝普钠(SNP, NO供体)的缓解作用, 以红芸豆品种‘红芸3号’为试材, 采用纸间发芽方法和水培法, 研究Cr和外源NO对红芸豆种子萌发、种胚和幼苗抗氧化系统、幼苗光合特性、营养元素吸收转运的影响。结果表明, 20 μmol·L−1 K2Cr2O7胁迫诱发红芸豆种胚和幼苗氧化损伤, 抑制幼苗对营养元素的吸收和转运、降低幼苗光合作用, 进而抑制红芸豆种子萌发和早期幼苗的生长。添加100 μmol·L−1 SNP (外源NO)可减轻Cr对种胚和幼苗的氧化胁迫伤害, 降低Cr在幼苗中的积累, 促进幼苗对营养元素的吸收和转运、增强幼苗光合作用等。种子萌发生长72 h时, 20 μmol·L−1 K2Cr2O7胁迫下添加SNP种胚H2O2 \mathrmO_2^- 和MDA含量分别比Cr胁迫处理降低36.15%、30.96%和34.97%; Cr胁迫下, 添加SNP幼苗生长10 d, 根系H2O2 \mathrmO_2^- 和MDA含量分别比Cr胁迫处理降低55.25%、58.39%和33.54%, 叶片H2O2 \mathrmO_2^- 和MDA含量分别比Cr胁迫处理降低27.71%、36.40%和53.84%, 根系活力比Cr胁迫处理升高59.16%; 幼苗根和叶中Cr含量比Cr胁迫处理降低69.14%和23.87%, 幼苗根中Mg、Fe、Cu、Zn等含量比Cr胁迫处理分别增加37.10%、17.97%、5.97%、13.60%, 幼苗叶中Mg、Fe、Cu、Zn等元素含量比Cr胁迫处理分别增加32.57%、29.44%、22.44%, 36.07%; 幼苗净光合速率、PSⅡ最大光合效率比Cr胁迫处理提高58.60%和11.27%。外源NO通过诱导种胚抗氧化酶活性提高、降低幼苗对Cr的吸收, 促进幼苗对营养元素的吸收和转运、增强幼苗光合作用, 缓解Cr对红芸豆种子萌发和早期幼苗生长的胁迫作用。

     

    Abstract: In order to study the stress effect of chromium (Cr) on seed germination and early seedling growth of red kidney bean and the alleviation effect of sodium nitroprusside (SNP,NO donor ), the red kidney bean variety ' Hongyun 3 ' was used as the test material. The effects of Cr and exogenous NO on seed germination, antioxidant system of embryo and seedling, photosynthetic characteristics of seedling, absorption and transport of nutrient elements of red kidney bean were studied by paper germination method and hydroponic method. 20 μmol∙L−1 K2Cr2O7 stress induced oxidative damage of red kidney bean embryos and seedlings, inhibited the absorption and transport of nutrients by seedlings, reduced the photosynthesis of seedlings, and then inhibited the germination of red kidney bean seeds and the growth of early seedlings. The addition of 100 μmol∙L−1 sodium nitroprusside (SNP, exogenous NO) could alleviate the oxidative stress damage of Cr to embryos and seedlings, reduce the accumulation of Cr in seedlings, promote the absorption and transport of nutrients in seedlings, and enhance the photosynthesis of seedlings. At 72 h of seed germination and growth, the contents of H2O2, \mathrmO_2^- and MDA in embryo decreased by 36.15 %, 30.96 % and 34.97%, respectively, compared with Cr stress treatment. Under Cr stress, the contents of H2O2, \mathrmO_2^- and MDA in the roots of seedlings treated with SNP decreased by 55.25%, 58.39% and 33.54%, respectively, compared with Cr stress treatment. The contents of H2O2, \mathrmO_2^- and MDA in leaves decreased by 27.71%, 36.40% and 53.84%, respectively, compared with Cr stress treatment. The root activity was 59.16% higher than that of Cr stress treatment. The content of Cr in roots and leaves of seedlings decreased by 69.14% and 23.87% compared with Cr stress treatment. The contents of Mg, Fe, Cu and Zn in roots of seedlings increased by 37.10%, 17.97%, 5.97% and 13.60% respectively compared with Cr stress treatment. The contents of Mg, Fe, Cu and Zn in leaves of seedlings increased by 32.57%, 29.44%, 22.44% and 36.07% respectively compared with Cr stress treatment. The net photosynthetic rate and PSII maximum photosynthetic efficiency of seedlings were 58.60% and 11.27% higher than those of Cr stress treatment. Exogenous NO alleviated the stress damage of Cr on seed germination and early seedling growth of red kidney bean by inducing the increase of antioxidant enzyme activity in embryo, reducing the absorption of Cr by seedlings, promoting the absorption and transport of nutrients by seedlings, and enhancing the photosynthesis of seedlings.

     

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