汤旭升, 刘文畅, 郭衍硕, 罗国芝, 谭洪新. 短期驯化对水芹耐低盐胁迫的影响[J]. 中国生态农业学报 (中英文), 2024, 32(0): 1−10. DOI: 10.12357/cjea.20240033
引用本文: 汤旭升, 刘文畅, 郭衍硕, 罗国芝, 谭洪新. 短期驯化对水芹耐低盐胁迫的影响[J]. 中国生态农业学报 (中英文), 2024, 32(0): 1−10. DOI: 10.12357/cjea.20240033
TANG X S, LIU W C, GUO Y S, LUO G Z, TAN H X. Effect of short-term domestication on the tolerance of Oenanthe javanica to low-salt stress[J]. Chinese Journal of Eco-Agriculture, 2024, 32(0): 1−10. DOI: 10.12357/cjea.20240033
Citation: TANG X S, LIU W C, GUO Y S, LUO G Z, TAN H X. Effect of short-term domestication on the tolerance of Oenanthe javanica to low-salt stress[J]. Chinese Journal of Eco-Agriculture, 2024, 32(0): 1−10. DOI: 10.12357/cjea.20240033

短期驯化对水芹耐低盐胁迫的影响

Effect of short-term domestication on the tolerance of Oenanthe javanica to low-salt stress

  • 摘要: 为探究低盐胁迫对水芹(Oenanthe javanica)生长的影响和短期驯化在提升其耐盐度方面的效果, 本研究评估并对比了54 d内, 盐度为2、4、6和8 PSU (Practical salinity units, 实用盐度)的低盐胁迫对驯化与未驯化水芹生长状态和生理生化的影响。在水培条件下, 以每3 d 提升2 PSU的方法对水芹进行驯化。结果显示, 经过54 d的胁迫, 短期驯化与未驯化的水芹最高可在6 PSU盐度条件下存活, 而盐度差异和短期驯化显著影响了水芹的生长和生化过程。胁迫盐浓度超过2 PSU时, 生长状态方面, 盐胁迫导致水芹的鲜重增长率、叶片相对含水量(RWC)、叶绿素(SPAD)和植物品质(总蛋白、维生素C和可溶性多糖)分别下降63.7%、5.8%、10.3%和13.4%以上, 而驯化后仅下降20.6%、3.8%、3.7%和2.1%以上; 生理生化方面, 盐胁迫导致水芹的丙二醛(MDA)、脯氨酸(Pro)、活性氧(ROS)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽过氧化物酶(GSH-PX)和过氧化氢酶(CAT)分别上升65.4%、33.0%、30.6%、25.2%、23.7%、14.9%、21.5%和16.3%以上, 而驯化后仅分别上升34.2%、66.8%、8.0%、42.5%、51.3%、42.5%、50.0%和45.0%以上; 盐胁迫导致叶片和根系K+/Na+比值分别下降89.4%和84.1%以上, 而驯化后仅分别下降64.8%和60.6%以上。综上, 低盐胁迫对水芹的生长及其生理生化反应产生不同程度的负面影响, 而短期驯化可减弱这种负面影响。

     

    Abstract: In order to investigate the effects of low salt stress on the growth of watercress (Oenanthe javanica) and the effectiveness of short-term domestication in enhancing its salinity tolerance, the present study evaluated and compared the effects of low salt stress with salinity levels of 2, 4, 6, and 8 PSU (Practical salinity units) on the growth status and physiological and biochemical characteristics of domesticated and undomesticated watercress over a 54 d period. Effects. The watercress was domesticated under hydroponic conditions by increasing 2 PSU every 3 days. The results showed that after 54 d of stress, the short-term domesticated and non-domesticated watercresses could survive up to 6 PSU salinity, and the growth and biochemical processes of the watercresses were significantly affected by the difference in salinity and the short-term domestication. In terms of growth status, salt stress caused watercress fresh weight growth rate, leaf relative water content (RWC), chlorophyll (SPAD), and plant quality (total protein, vitamin C, and soluble polysaccharides) to decrease by more than 63.7%, 5.8%, 10.3%, and 13.4%, respectively, at a stress salt concentration exceeding 2 PSU, whereas domestication caused them to decrease by only 20.6%, 3.8%, 3.7%, and 2.1% or more; in terms of physiology and biochemistry, salt stress resulted in an increase in malondialdehyde (MDA), proline (Pro), reactive oxygen species (ROS), superoxide dismutase (SOD), peroxidase (POD), ascorbic acid peroxidase (APX), glutathione peroxidase (GSH-PX), and catalase (CAT), respectively, in watercress by 65.4%, 33.0%, 30.6%, 25.2%, 23.7%, 14.9%, 21.5% and 16.3%, respectively, whereas only 34.2%, 66.8%, 8.0%, 42.5%, 51.3%, 42.5%, 50.0% and 45.0%, respectively, were increased by domestication; Salt stress resulted in the decreases of K+/Na+ ratio in leaves and roots by 89.4% and Salt stress caused the K+/Na+ ratios of leaves and roots to decrease by more than 89.4% and 84.1%, respectively, whereas they only decreased by more than 64.8% and 60.6%, respectively, after domestication. In conclusion, low salt stress had different negative effects on the growth of watercress and its physiological and biochemical responses, but short-term domestication could reduce the negative effects.

     

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