车延静, 谷思玉, 王紫颖, 白雪燕, 冯景翊, 张伟健, 李宇航. 保护性耕作下不同区域农田黑土溶解性有机碳荧光特性分析[J]. 中国生态农业学报 (中英文), 2024, 32(10): 1−12. DOI: 10.12357/cjea.20240146
引用本文: 车延静, 谷思玉, 王紫颖, 白雪燕, 冯景翊, 张伟健, 李宇航. 保护性耕作下不同区域农田黑土溶解性有机碳荧光特性分析[J]. 中国生态农业学报 (中英文), 2024, 32(10): 1−12. DOI: 10.12357/cjea.20240146
CHE Y J, GU S Y, WANG Z Y, BAI X Y, FENG J Y, ZHANG W J, LI Y H. Analysis of DOC fluorescence spectral structure of black soil in different regions under conservation tillage[J]. Chinese Journal of Eco-Agriculture, 2024, 32(10): 1−12. DOI: 10.12357/cjea.20240146
Citation: CHE Y J, GU S Y, WANG Z Y, BAI X Y, FENG J Y, ZHANG W J, LI Y H. Analysis of DOC fluorescence spectral structure of black soil in different regions under conservation tillage[J]. Chinese Journal of Eco-Agriculture, 2024, 32(10): 1−12. DOI: 10.12357/cjea.20240146

保护性耕作下不同区域农田黑土溶解性有机碳荧光特性分析

Analysis of DOC fluorescence spectral structure of black soil in different regions under conservation tillage

  • 摘要: 土壤溶解性有机碳(DOC)组成、含量和周转速率直接影响微生物群落组成及活性, 从而影响土壤有机碳的矿化过程。本文研究不同区域保护性耕作措施对土壤DOC荧光特性的影响, 以揭示保护性耕作对黑土碳库稳定性的影响, 为科学选择耕作方式提供理论依据。以黑龙江省克山(厚层黑土)和吉林省公主岭(薄层黑土)两区域耕作定位试验农田黑土为研究对象, 设置免耕(NT)、免耕+秸秆还田(NR)、深松(ST)和深松+秸秆还田(SR) 4个保护性耕作处理, 以传统翻耕(CT)、翻耕+秸秆还田(CR)为对照, 共6个处理, 采用荧光光谱法, 分析不同耕作处理下厚层和薄层黑土区0~20 cm土层农田土壤DOC含量、组成及结构特征。结果表明: 厚层黑土区, 土壤总有机碳(TOC)含量表现为SR>NR>CR>ST>NT>CT, DOC含量为SR>ST>NT>NR>CR>CT, SR处理土壤TOC和DOC含量均最高, 与CR相比分别显著增加6.88%和25.61% (P<0.05); 薄层黑土区, NR处理土壤TOC和DOC含量均最高, 与CR相比, DOC含量显著增加21.30% (P<0.05)。平行因子分析结果将厚层和薄层黑土区DOC均分为3个组分, C1组分为色氨酸类蛋白质, C2组分为类富里酸物质, C3组分为类腐殖质物质。荧光指数分析表明, 厚层和薄层黑土区农田黑土均受内外源共同作用。厚层黑土区, SR处理自生源特性最低, 受外源输入影响大, 腐殖化系数最高, DOC结构更稳定; 薄层黑土区, NR处理自生源特性最低, 微生物贡献率低, 主要为外源秸秆输入, DOC结构稳定不易被微生物分解利用。综上, 保护性耕作措施对厚层和薄层两区域农田黑土TOC和DOC均具有一定的提升作用, 深松秸秆还田措施更有利于厚层黑土TOC与DOC的积累及碳库稳定, 免耕秸秆还田更利于薄层黑土有机碳的提升与稳定。

     

    Abstract: The composition, content and turnover rate of soil dissolved organic carbon (DOC) directly affect the composition and activity of microbial communities, thus influencing the mineralization process of soil organic carbon. The present study investigated the effects of conservation tillage practices on the fluorescence properties of soil DOC in different regions, to reveal the effects of conservation tillage on the stability of black soil carbon pools. Furthermore, this research provides a theoretical basis for the scientific selection of tillage methods. In this research, four conservation tillage treatments, including no-tillage (NT), no-tillage + straw returning (NR), subsoiling (ST) and subsoiling + straw returning (SR), were set up in Keshan, Heilongjiang Province (black thick-layer soil ) and Gongzhuling, Jilin Province (black thin-layer soil). The traditional tillage (CT) and tillage + straw returning (CR) were used as the controls. There were 6 treatments in total. Fluorescence spectroscopy was used to analyze the content, composition, and structural characteristics of farmland soil DOC in the 0~20 cm in black thick-layer soil and black thin-layer soil areas under different tillage treatments. The results showed that in black thick-layer soil area, soil TOC content showed SR>NR>CR>ST>NT>CT, DOC content was SR>ST>NT>NR>CR>CT. Both soil TOC and DOC content were the highest in the SR treatment, which were significantly increased (P<0.05) by 6.88% and 25.61%, respectively, compared with the CR treatment. In black thin-layer soil area, the contents of TOC and DOC in NR treatment were the highest, and the DOC content significantly increased (P < 0.05) by 21.30% compared to CR. The results of parallel factor analysis showed that DOC in black thick-layer soil and black thin-layer soil areas were divided into three components: C1 was composed of tryptophan proteins, C2 was a fulvic acid-like substance, and C3 was a humus-like substance. Fluorescence index analysis indicated that in black thick-layer soil and black thin-layer soil areas were affected by both internal and external influences. In black thick-layer soil area, the SR treatment had the lowest autogenic characteristics, which was greatly affected by exogenous input, with the highest humification coefficient, and a more stable DOC structure; In black thin-layer soil area, the NR treatment had the lowest autochthonous source characteristics, low microbial contribution, mainly from exogenous straw input, with stable DOC structure not easily utilized by microbial decomposition. In conclusion, conservation tillage measures have a certain enhancing effect on both TOC and DOC in both thick and thin layers regions of agricultural fields; The SR treatment was more conducive to the accumulation of TOC and DOC and the stabilization of carbon pools in black thick-layer soil area, and the NR treatment was more advantageous for the enhancement and stabilization of organic carbon in black thin-layer soil.

     

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