燕平梅, 赵文婧, 单树花, 宋敏丽, 孙毅. 转基因抗矮花叶病玉米及其亲本生理特性的对比研究[J]. 中国生态农业学报(中英文), 2012, 20(6): 772-776. DOI: 10.3724/SP.J.1011.2012.00772
引用本文: 燕平梅, 赵文婧, 单树花, 宋敏丽, 孙毅. 转基因抗矮花叶病玉米及其亲本生理特性的对比研究[J]. 中国生态农业学报(中英文), 2012, 20(6): 772-776. DOI: 10.3724/SP.J.1011.2012.00772
YAN Ping-Mei, ZHAO Wen-Jing, SHAN Shu-Hua, SONG Min-Li, SUN Yi. Seedling physiological characteristics of genetically modified maize variety with dwarf mosaic virus resistant genes and its parent[J]. Chinese Journal of Eco-Agriculture, 2012, 20(6): 772-776. DOI: 10.3724/SP.J.1011.2012.00772
Citation: YAN Ping-Mei, ZHAO Wen-Jing, SHAN Shu-Hua, SONG Min-Li, SUN Yi. Seedling physiological characteristics of genetically modified maize variety with dwarf mosaic virus resistant genes and its parent[J]. Chinese Journal of Eco-Agriculture, 2012, 20(6): 772-776. DOI: 10.3724/SP.J.1011.2012.00772

转基因抗矮花叶病玉米及其亲本生理特性的对比研究

Seedling physiological characteristics of genetically modified maize variety with dwarf mosaic virus resistant genes and its parent

  • 摘要: 为了探明转基因玉米的生理特性, 本文研究了转基因抗矮花叶病玉米和亲本玉米(非转基因玉米)的发芽能力及苗期第1真叶的叶绿素含量和淀粉酶、蛋白酶、过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性以及3种保护酶同工酶的酶谱。转基因玉米与非转基因玉米的发芽能力差异不显著, 叶绿素a和叶绿素a+b含量显著高于非转基因玉米。转基因玉米淀粉酶活性和蛋白酶活性显著高于非转基因玉米。转基因玉米与非转基因玉米胚芽中POD、CAT和SOD酶活力均无显著性差异, 转基因玉米第1真叶中3种保护酶活性显著高于非转基因玉米。转基因玉米和非转基因玉米胚芽中检测到POD的3种同工酶POD-1、POD-3和POD-4, 非转基因玉米第1真叶中检测到4种同工酶POD-1、POD-2、POD-3和POD-4; 转基因玉米第1真叶还含有POD-5。转基因玉米和非转基因玉米胚芽和第1真叶中都有4种CAT同工酶CAT-1、CAT-2、CAT-3和CAT-4。转基因玉米和非转基因玉米胚芽中的SOD共有两种同工酶, 分别为SOD-1和SOD-2; 转基因玉米和非转基因玉米第1真叶中共检测到7种SOD同工酶, 分别为SOD-1、SOD-2、SOD-3、SOD-4、SOD-5、SOD-6和SOD-7。综上所述, 转基因抗矮花叶病玉米发芽能力及胚芽中保护酶活性与非转基因玉米无显著差异, 转基因玉米第1真叶中3种保护酶活性显著高于非转基因玉米。

     

    Abstract: In order to verify the physiological characteristics of genetically modified maize, this paper studied the germination ability, chlorophyll content and activities of amylase, protease, peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) of genetically modified maize with dwarf mosaic virus resistance genes and its parent. The paper also analyzed zymograms of three protective enzymes. Germination abilities of genetically modified maize and its parent were not significantly different. Chlorophyll-a and chlorophyll-a+b contents were significantly higher in the modified maize than in the parent maize. Amylase and protease activities of genetically modified maize were significantly higher than those of the parent. POD, CAT and SOD enzyme activities of the germs of genetically modified maize and the parent were not significantly different. Three protective enzymes activities of the first true leaf of genetically modified maize were significantly higher than those of the parents. Three isozymes of POD (POD-1, POD-3 and POD-4) were detected both in the germs of genetically modified maize and the parent. Four isozymes of POD (POD-1, POD-2, POD-3 and POD-4) were detected in the first true leaf of the parent, and five isozymes of POD (POD-1, POD-2, POD-3, POD-4 and POD-5) in the first true leaf of genetically modified maize. However, four CAT isozymes (CAT-1, CAT-2, CAT-3 and CAT-4) exited in the germs and the first true leaf of both the genetically modified maize and the parents. Isozymes of SOD in germs of genetically modified maize and the parents had two bands consisting of SOD-1 and SOD-2. However, seven bands (SOD-1, SOD-2, SOD-3, SOD-4, SOD-5, SOD-6, and SOD-7) were noted in the first true leaf both of the genetically modified maize and the parents. The results showed that physiological indexes of genetically modified maize and the parent were different. Three protective enzymes activities in the first true leaf of genetically modified maize were significantly higher than those of the parent.

     

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