培养基成分对沙打旺组培根生长及分泌物的影响研究

Effect of medium composition on the growth and secretion of cultured root of Astragalus adsurgens Pall

摘要: 研究表明培养基成分可影响沙打旺组培根生长和根分泌物的产生。适宜沙打旺组培根生长的改良B₅培养基组分为（以1L标准B₅培养基为基础调节，下同）KNO₃2660.5mg、(NH₄)₂SO₄84.1mg、CaCl₂·2H₂O75.0mg、MgSO₄·7H₂O125.0mg、FeSO₄·7H₂O13.9mg、MnSO₄·4H₂O5.0mg和萘乙酸（NAA）0.6mg。组培根分泌物对沙打旺和小麦胚根生长抑制作用最强的培养基组分为KNO₃4565.9mg、(NH₄)₂SO₄48.1mg、MnSO₄·4H₂O5.0mg、肌醇150.0mg、烟酸1.5mg、盐酸吡哆辛1.5mg、盐酸硫胺15.0mg、NAA0.8mg和蔗糖50.0g。

Abstract: The growth and secretion of cultured root of Astragalus adsurgens Pall.are affected by the medium composition.One litre of modified B₅medium of stimulating the growth of the cultured root of A.adsurgens contains KNO₃2660.5mg，(NH₄)₂SO₄ 84.1mg，CaCl₂·2H₂O 75.0mg，MgSO₄·7H₂O 125.0mg，FeSO₄·7H₂O 13.9mg，MnSO₄·4H₂O 5.0mg，α-Naphthylacetic acid（NAA）0.6mg.One litre of modified B₅ medium of high-stayer-producing A.adsurgens root culture consists of KNO₃4565.9mg，(NH₄)₂SO₄48.1mg，MnSO₄·4H₂O5.0mg，inositol 150.0mg，nicotinic acid 1.5mg，vitamin B₆ hydrochloride 1.5mg，vitamin B₁ 15.0mg，NAA0.8mg，and sucrose 50.0g.