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Reference Text:WANG Kaili,GAO Yanzhao,LI Shan,ZHANG Menglu,WU Zhihao,LIU Liantao,SUN Hongchun,LI Cundong,ZHANG Yongjiang.Response of leaf stomata and photosynthetic parameters to short-term drought stress in cotton (Gossypium hirsutum L.)[J].Chinese Journal of Eco-Agriculture,2019,27(6):901-907
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Response of leaf stomata and photosynthetic parameters to short-term drought stress in cotton (Gossypium hirsutum L.)
WANG Kaili, GAO Yanzhao, LI Shan, ZHANG Menglu, WU Zhihao, LIU Liantao, SUN Hongchun, LI Cundong, ZHANG Yongjiang
College of Agronomy, Hebei Agricultural University/Key Laboratory of Crop Growth Regulation of Hebei Province, Baoding 071001, China
Abstract:  Studying the response of stomata to drought stress can help to understand the behavior of stomatal regulation and improve water utilization efficiency of crops. This study was conducted in an artificial climate chamber using the cotton cultivar ‘Guoxin 9’. Four concentrations of PEG 6000[0 (control), 1.5%, 3%, and 4.5%] were added into the nutrient solution. Stomatal morphological parameters, gas exchange parameters, and chlorophyll fluorescence kinetic parameters were measured every two days from the first day to the seventh day of treatment. The results showed that with the elongation of treatment time, the values of leaf water potential, stomatal length, stomatal width, stomatal aperture, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), intercellular CO2 concentration (Ci), maximal photochemical efficiency (Fv/Fm), and actual photochemical production quantum (Yield) all decreased to some extent, whereas stomatal density and non-photochemical quenching coefficient (NPQ) increased. Similar trends were observed in different treatments with an increase in drought stress. After 5 d and 7 d of treatment, leaf water potential, stomatal length, stomatal width, Pn, Gs, Tr, and Ci with 1.5%, 3%, and 4.5% PEG 6000 were significantly different from those with the control (P <0.05). The magnitudes of the declines in leaf stomatal length, Gs, and Tr were 1.17%-2.61%, 61.62%-69.09%, and 37.62%-67.48%, respectively. Correlation analysis revealed that significant positive correlations existed among leaf stomatal length, width, and aperture (P<0.01). Stomatal width was not significantly correlated with stomatal density. Stomatal length, width, and aperture were positively correlated with Pn and Yield (P<0.01), negatively correlated with NPQ (P<0.01), and not significantly correlated with Gs, Tr, or Fv/Fm. These results suggested that the simulated drought induced by PEG 6000 may result in cotton stomatal closure and an increase in stomatal density, along with decreases in net photosynthetic rate and stomatal conductance. Compared with Yield and NPQ, Fv/Fm was less sensitive to drought stress in cotton.
Keyword:  Cotton  Stomatal morphology  Stomatal density  Photosynthetic characteristics  Drought stress  Chlorophyll fluorescence