Cloning and sequence analysis of chloromuconate cycloisomerase gene

A 2,4-dichlorophenol degrading Pseudomonas strain GT241-1 was isolated from a soil sample. The chloromuconate cycloisomerase gene, designated as dcpC which encodes chloromuconate cycloisomerase involved in transforming 2,4-dichloro-cis,cis-muconate into trans-2-chlorodienelactone,was cloned from this bacterium strain.The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southern blot and to screen the aim transformant by dot blotting.Sequencing results show that the length of dcpC is 1110bp.The sequence analysis of dcpC and the deduced amino acid show that dcpC belongs to chloromuconate cycloisomerase gene family and is different from other genes in the gene family.