Abstract: Aging tea trees, degradation of soil fertility in tea gardens, and poor soil biodiversity have become important factors that limit the yield and quality of organic tea. Earthworms play an important role in the regulation of soil ecosystem services. The inoculation of two widely reared earthworm pioneer species was tested using field experiments on a tea plantation in China. Field experiments were conducted to explore the effects of inoculation with different types of earthworm on soil fungal communities in organic tea gardens. Four treatments were set up, including: Eisenia fetida inoculation with a inoculation density of 300 individual·m⁻² (E), Metaphire guillelmi inoculation with a inoculation density of 100 individual·m⁻² (P), simultaneous inoculation of Eisenia fetida with a inoculation density of 150 individual·m⁻² and Metaphire guillelmi inoculation with a inoculation density of 50 individual·m⁻² (EP), and control (CK). Soil fungal communities were investigated using high-throughput sequencing. The results showed that the community structure and functional groups of the soil fungi changed significantly. Compared with CK treatment, inoculation with earthworms significantly increased the number of operational taxonomic unit (OUT) in the soil fungi (excluding EP treatment), and the number of dominant OTU increased from 100 to 269. Compared with CK treatment, inoculating earthworms increased the α diversity of soil fungal community; Shannon, Simpson, and Fisher indices under the treatments with single type earthworm inoculation also increased (P<0.05). Ascomycota and Basidiomycota were the dominant fungal groups in tea garden soil. Compared with CK treatment, Ascomycota further increased under the treatments with single type earthworm inoculation (E and P treatments) and decreased slightly under EP treatment. Compared with CK treatment, increase in relative abundance of Mortierellomycota under EP treatment (10.16%) was the largest. EP treatment showed the best population uniformity, and more undefined fungi appeared with a proportion of 29.61%. Xylariales decomposes litter, thus the EP treatment exhibited a stronger ability to decompose dead branches and leaves than single type earthworm inoculation treatment, which is more beneficial for increasing soil nutrients in organic tea gardens. Compared with conventional organic tea gardens, inoculation with earthworms reduced the relative abundance of saprotrophic fungi while improving the growth of pathotroph-saprotroph-symbiotroph fungi. In addition, EP treatment promoted the growth of saprotroph-symbiotroph fungi. Generally, in biologically and physically degraded soil systems, mutual promotion between earthworm species outweighs competition. Mixed inoculation of different types of earthworms affected pathogen control, which can help control potential pathogens, thereby providing a sustainable solution for improving soil biodiversity and enhancing soil quality in organic tea gardens. Inoculating earthworms is an effective and feasible ecological measure for improving soil quality in organic tea gardens.