Volume 27 Issue 6
Jun.  2019
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WANG Kaili, GAO Yanzhao, LI Shan, ZHANG Menglu, WU Zhihao, LIU Liantao, SUN Hongchun, LI Cundong, ZHANG Yongjiang. Response of leaf stomata and photosynthetic parameters to short-term drought stress in cotton (Gossypium hirsutum L.)[J]. Chinese Journal of Eco-Agriculture, 2019, 27(6): 901-907. doi: 10.13930/j.cnki.cjea.180928
Citation: WANG Kaili, GAO Yanzhao, LI Shan, ZHANG Menglu, WU Zhihao, LIU Liantao, SUN Hongchun, LI Cundong, ZHANG Yongjiang. Response of leaf stomata and photosynthetic parameters to short-term drought stress in cotton (Gossypium hirsutum L.)[J]. Chinese Journal of Eco-Agriculture, 2019, 27(6): 901-907. doi: 10.13930/j.cnki.cjea.180928

Response of leaf stomata and photosynthetic parameters to short-term drought stress in cotton (Gossypium hirsutum L.)

doi: 10.13930/j.cnki.cjea.180928

the National Key Research and Development Program of China 2017YFD0201900

the Natural Science Foundation of Hebei Province C2016204004

the Innovation Training Program for College Students in Hebei 201710086047

the Modern Technology System of Agricultural Industry in Hebei HBCT2018040201

More Information
  • Corresponding author: ZHANG Yongjiang, E-mail:yongjiangzh@sina.com
  • Received Date: 2018-10-19
  • Accepted Date: 2018-11-26
  • Publish Date: 2019-06-01
  • Studying the response of stomata to drought stress can help to understand the behavior of stomatal regulation and improve water utilization efficiency of crops. This study was conducted in an artificial climate chamber using the cotton cultivar 'Guoxin 9'. Four concentrations of PEG 6000[0 (control), 1.5%, 3%, and 4.5%] were added into the nutrient solution. Stomatal morphological parameters, gas exchange parameters, and chlorophyll fluorescence kinetic parameters were measured every two days from the first day to the seventh day of treatment. The results showed that with the elongation of treatment time, the values of leaf water potential, stomatal length, stomatal width, stomatal aperture, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), intercellular CO2 concentration (Ci), maximal photochemical efficiency (Fv/Fm), and actual photochemical production quantum (Yield) all decreased to some extent, whereas stomatal density and non-photochemical quenching coefficient (NPQ) increased. Similar trends were observed in different treatments with an increase in drought stress. After 5 d and 7 d of treatment, leaf water potential, stomatal length, stomatal width, Pn, Gs, Tr, and Ci with 1.5%, 3%, and 4.5% PEG 6000 were significantly different from those with the control (P < 0.05). The magnitudes of the declines in leaf stomatal length, Gs, and Tr were 1.17%-2.61%, 61.62%-69.09%, and 37.62%-67.48%, respectively. Correlation analysis revealed that significant positive correlations existed among leaf stomatal length, width, and aperture (P < 0.01). Stomatal width was not significantly correlated with stomatal density. Stomatal length, width, and aperture were positively correlated with Pn and Yield (P < 0.01), negatively correlated with NPQ (P < 0.01), and not significantly correlated with Gs, Tr, or Fv/Fm. These results suggested that the simulated drought induced by PEG 6000 may result in cotton stomatal closure and an increase in stomatal density, along with decreases in net photosynthetic rate and stomatal conductance. Compared with Yield and NPQ, Fv/Fm was less sensitive to drought stress in cotton.
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  • [1]
    HETHERINGTON A M, WOODWARD F I. The role of stomata in sensing and driving environmental change[J]. Nature, 2003, 424(6951):901-908 doi: 10.1038/nature01843
    李芳兰, 包维楷.植物叶片形态解剖结构对环境变化的响应与适应[J].植物学报, 2005, 22(S1):118-127 http://www.cqvip.com/QK/96906X/2005B08/21139456.html

    LI F L, BAO W K. Responses of the morphological and anatomical structure of the plant leaf to environmental change[J]. Chinese Bulletin of Botany, 2005, 22(S1):118-127 http://www.cqvip.com/QK/96906X/2005B08/21139456.html
    CASSON S A, HETHERINGTON A M. Environmental regulation of stomatal development[J]. Current Opinion in Plant Biology, 2010, 13(1):90-95 doi: 10.1016/j.pbi.2009.08.005
    于海秋, 王晓磊, 蒋春姬, 等.土壤干旱下玉米幼苗解剖结构的伤害进程[J].干旱地区农业研究, 2008, 26(5):143-147 http://d.old.wanfangdata.com.cn/Periodical/ghdqnyyj200805030

    YU H Q, WANG X L, JIANG C J, et al. Injured process on anatomical structure of maize seedling under soil drought[J]. Agricultural Research in the Arid Areas, 2008, 26(5):143-147 http://d.old.wanfangdata.com.cn/Periodical/ghdqnyyj200805030
    张志芬, 刘景辉, 付晓峰, 等.干旱胁迫对燕麦叶片气孔和叶肉细胞超微结构的影响[J].麦类作物学报, 2017, 37(9):1216-1223 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201709012

    ZHANG Z F, LIU J H, FU X F, et al. Effect of drought stress on stomata and ultrastructure of mesophyll cells of oat leaf[J]. Journal of Triticeae Crops, 2017, 37(9):1216-1223 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201709012
    ZHANG Y L, HU Y Y, LUO H H, et al. Two distinct strategies of cotton and soybean differing in leaf movement to perform photosynthesis under drought in the field[J]. Functional Plant Biology, 2011, 38(7):567-575 doi: 10.1071/FP11065
    王春艳, 庞艳梅, 李茂松, 等.干旱胁迫对大豆气孔特征和光合参数的影响[J].中国农业科技导报, 2013, 15(1):109-115 http://d.old.wanfangdata.com.cn/Periodical/zgnykjdb201301024

    WANG C Y, PANG Y M, LI M S, et al. Effects of drought stress on soybean stomatal characteristics and photosynthetic parameter[J]. Journal of Agricultural Science and Technology, 2013, 15(1):109-115 http://d.old.wanfangdata.com.cn/Periodical/zgnykjdb201301024
    李平, 张永江, 刘连涛, 等.水分胁迫对棉花幼苗水分利用和光合特性的影响[J].棉花学报, 2014, 26(2):113-121 doi: 10.3969/j.issn.1002-7807.2014.02.003

    LI P, ZHANG Y J, LIU L T, et al. Effects of water stress on water utilization and leaf photosynthetic characteristics in cotton (Gossypium hirsutum L.) seedlings[J]. Cotton Science, 2014, 26(2):113-121 doi: 10.3969/j.issn.1002-7807.2014.02.003
    MASSACCI A, NABIEV S M, PIETROSANTI L, et al. Response of the photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging[J]. Plant Physiology and Biochemistry, 2008, 46(2):189-195 doi: 10.1016/j.plaphy.2007.10.006
    SNIDER J L, OOSTERHUIS D M, COLLINS G D, et al. Field-acclimated Gossypium hirsutum cultivars exhibit genotypic and seasonal differences in photosystem Ⅱ thermostability[J]. Journal of Plant Physiology, 2013, 170(5):489-496 doi: 10.1016/j.jplph.2012.11.004
    李东晓, 李存东, 孙传范, 等.干旱对棉花主茎叶片内源激素含量与平衡的影响[J].棉花学报, 2010, 22(3):231-235 doi: 10.3969/j.issn.1002-7807.2010.03.007

    LI D X, LI C D, SUN C F, et al. The effects of drought on endogenous hormone contents and balance in main stem leaves of cotton[J]. Cotton Science, 2010, 22(3):231-235 doi: 10.3969/j.issn.1002-7807.2010.03.007
    罗宏海, 张亚黎, 张旺锋, 等.新疆滴灌棉花花铃期干旱复水对叶片光合特性及产量的影响[J].作物学报, 2008, 34(1):171-174 doi: 10.3321/j.issn:0496-3490.2008.01.026

    LUO H H, ZHANG Y L, ZHANG W F, et al. Effects of rewatering after drought stress on photosynthesis and yield during flowering and boll-setting stage of cotton under-mulch-drip irrigation in Xinjiang[J]. Acta Agronomica Sinica, 2008, 34(1):171-174 doi: 10.3321/j.issn:0496-3490.2008.01.026
    MAXWELL K, JOHNSON G N. Chlorophyll fluorescence-A practical guide[J]. Journal of Experimental Botany, 2000, 51(345):659-668 doi: 10.1093/jexbot/51.345.659
    CASSON S, GRAY J E. Influence of environmental factors on stomatal development[J]. New Phytologist, 2008, 178(1):9-23 doi: 10.1111/j.1469-8137.2007.02351.x
    杨再强, 谭文, 刘朝霞, 等.土壤水分胁迫对设施番茄叶片气孔特性的影响[J].生态学杂志, 2015, 34(5):1234-1240 http://d.old.wanfangdata.com.cn/Periodical/stxzz201505008

    YANG Z Q, TAN W, LIU Z X, et al. Effect of soil water stress on stomatal characters of greenhouse tomato leaves[J]. Chinese Journal of Ecology, 2015, 34(5):1234-1240 http://d.old.wanfangdata.com.cn/Periodical/stxzz201505008
    张德巧, 徐增莱, 褚晓芳, 等.蓝莓叶片与抗旱性相关的解剖结构指标研究[J].果树学报, 2008, 25(6):864-867 http://www.cnki.com.cn/Article/CJFDTOTAL-GSKK200806024.htm

    ZHANG D Q, XU Z L, CHU X F, et al. Study on leaf anatomical structure indexes related to drought resistance of blueberry[J]. Journal of Fruit Science, 2008, 25(6):864-867 http://www.cnki.com.cn/Article/CJFDTOTAL-GSKK200806024.htm
    FRASER L H, GREENALL A, CARLYLE C, et al. Adaptive phenotypic plasticity of Pseudoroegneria spicata:Response of stomatal density, leaf area and biomass to changes in water supply and increased temperature[J]. Annals of Botany, 2009, 103(5):769-775 doi: 10.1093/aob/mcn252
    XU Z Z, ZHOU G S. Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass[J]. Journal of Experimental Botany, 2008, 59(12):3317-3325 doi: 10.1093/jxb/ern185
    李中华, 刘进平, 谷海磊, 等.干旱胁迫对植物气孔特性影响研究进展[J].亚热带植物科学, 2016, 45(2):195-200 doi: 10.3969/j.issn.1009-7791.2016.02.021

    LI Z H, LIU J P, GU H L, et al. Review on the effects of drought stress on plant stomatal characteristics[J]. Subtropical Plant Science, 2016, 45(2):195-200 doi: 10.3969/j.issn.1009-7791.2016.02.021
    HUGHES J, HEPWORTH C, DUTTON C, et al. Reducing stomatal density in barley improves drought tolerance without impacting on yield[J]. Plant Physiology, 2017, 174(2):776-787 doi: 10.1104/pp.16.01844
    TOMBESI S, NARDINI A, FRIONI T, et al. Stomatal closure is induced by hydraulic signals and maintained by ABA in drought-stressed grapevine[J]. Scientific Reports, 2015, 5:12449 doi: 10.1038/srep12449
    ENNAHLI S, EARL H J. Physiological limitations to photosynthetic carbon assimilation in cotton under water stress[J]. Crop Science, 2005, 45(6):2374-2382 doi: 10.2135/cropsci2005.0147
    AHMED S, NAWATA E, HOSOKAWA M, et al. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging[J]. Plant Science, 2002, 163(1):117-123 doi: 10.1016/S0168-9452(02)00080-8
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